张瑶,田玉玲,王慧玲,王娜,王利武,谢智博,李汶霞,王雅各,宋金华,夏百成,孙馨,张燕,纪龙

• 1:山东第一医科大学(山东省医学科学院)
• 2:中国疾病预防控制中心病毒病预防控制所国家卫生健康委医学病毒和病毒病重点实验室传染病溯源预警与智能决策全国重点实验室
• 3:长春市儿童医院

摘要(Abstract)：

目的 了解2019—2022年吉林省长春市某哨点医院儿童急性呼吸道感染（acute respiratory infections,ARIs）病例中肠道病毒的流行情况及型别组成。方法 采集2019年6月—2022年12月长春市儿童医院ARIs患儿的咽拭子样本，并收集患儿临床信息，用描述性分析方法对肠道病毒感染病例的性别、年龄和季节分布情况进行分析。采用实时荧光反转录聚合酶链反应（real-time quantitative reverse transcription PCR,real-time qRT-PCR）技术检测肠道病毒，使用反转录聚合酶链反应（reverse transcription PCR,RT-PCR）技术扩增肠道病毒VP1全长并进行系统进化分析。结果 共采集2 707例ARIs病例的咽拭子样本，检出肠道病毒阳性样本65例，检出率为2.40%。65份阳性样本中，32.31%(21/65)为混合感染，以肠道病毒和鼻病毒的双重混合感染最为常见，占肠道病毒阳性病例的10.77%(7/65)。2019—2022年春季、夏季、秋季和冬季肠道病毒的检出率分别为0、6.91%(40/579)、2.90%(21/725)和0.45%(4/894)，不同季节肠道病毒检出率差异有统计学意义（χ~2=78.036,P<0.01），夏季检出率最高，秋季次之。本研究共鉴定出10种型别肠道病毒，分别为CVA2、CVA4、CVA5、CVA6、CVA10、CVA16、CVB3、E11、E18和EV-D68，其中CVA4检出最多，占比为44.82%(26/58)，其余型别检出占比为1.72%～12.07%。结论 2019—2022年长春市儿童ARIs病例中肠道病毒夏秋季检出率较高，肠道病毒感染以EV-A组为主。

关键词(KeyWords)： 急性呼吸道感染;肠道病毒;基因分型;流行病学;住院病例

Abstract:

Keywords:

基金项目(Foundation): 国家“十三五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项（2018ZX10713002）

作者(Author): 张瑶,田玉玲,王慧玲,王娜,王利武,谢智博,李汶霞,王雅各,宋金华,夏百成,孙馨,张燕,纪龙

DOI: 10.16505/j.2095-0136.2024.2006

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