普伟,赵福琴,柏桦,姜黎黎,田炳均,伏晓庆,罗春蕊

• 1:蒙自市疾病预防控制中心
• 2:昆明医科大学公共卫生学院
• 3:云南省疾病预防控制中心急性传染病防制所

摘要(Abstract)：

目的 对2021年云南省红河州手足口病（hand,foot and mouth disease,HFMD）病例标本中分离的2株新型肠道病毒EV-C96的基因特征进行分析，为今后对该病毒的防控提供分子流行病学数据。方法 收集2021年1—12月红河州手足口病病例的粪便和咽拭子标本，从处理后的标本中提取病毒RNA，采用实时荧光定量反转录聚合酶链反应（real-time quantitative reverse transcription PCR,real-time qRT-PCR）法进行病毒鉴定。对鉴定出的其他肠道病毒进行基因测序定型。用肠道病毒特异性引物扩增VP1区基因，用DNAStar软件中的Seqman程序对病毒基因进行编辑、整理，得到其VP1区序列。按文献下载EV-C96病毒VP1基因代表株参考序列，用Mega 5.2软件进行基因特征分析。结果 2021年红河州共报告手足口病病例6 017例，发病率为171.73/10万，其中，经real-time qRT-PCR法确诊的429例手足口病病例中，EV-A71感染57例，占13.29%(57/429),CVA16感染137例，占31.93%(137/429)，其他肠道病毒感染235例，占54.78%(235/429)，用基因测序法从235例其他肠道病毒感染病例样本中检测到2株EV-C96，占0.85%(2/235)。基因特征分析表明这2株EV-C96病毒属C基因型。结论 2021年云南省红河州手足口病病原体以其他肠道病毒为主，其中2株为EV-C96，属C基因型。这是云南省首次从手足口病病例中检测到新型肠道病毒EV-C96。

关键词(KeyWords)： 手足口病;肠道病毒C组96型;VP1区基因;基因特征分析

Abstract:

Keywords:

基金项目(Foundation): 国家“艾滋病和病毒性肝炎等重大传染病防治”科技重大专项（2017ZX10103010）

作者(Author): 普伟,赵福琴,柏桦,姜黎黎,田炳均,伏晓庆,罗春蕊

DOI: 10.16505/j.2095-0136.2024.2003

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